بررسی اثرات تکثیری هورمون تیروئیدی T4 بر سلول های گلیوبلاستومای مغزی U87

Background: Considering the effect of thyroid hormone on cancer cells, as well as the development of hormonal therapies for cancer cells. The aim of this study was to investigate the effects of cytotoxic thyroid hormones T4 on proliferation of brain glioblastoma cells (U87) in cell culture. Methods: In this laboratory experimental study, U87 cells were randomly divided into control group and groups exposed to doses 0.1, 0.5, 1, 5, 10, 20, 40, 50 Lambda (λ) of thyroid hormones T4. The toxic effect of hormones was measured using MTT assay method. The data were statistically analyzed between groups using ANOVA and SPSS. Results: The results show that the survival of U87 cells at doses of 0.5 λ of thyroid hormone T4 at 3 times of 24, 48 and 72 hours compared with other groups in different doses had the least toxic effect. Also survival of cells at 5 λ dose of thyroid hormone T4 at 24, 48 and 72 hours compared to other groups had the highest toxicity. Conclusion: Toxicity of glioblastoma cells is not dependent on thyroid hormone (T4), and results of this study showed that thyroid hormone does not affect U87 glioblastoma cells.